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Fig. 4. Segregation of a nucleolar marker (Nuc1p-GFP) in clp1
cells. Time-lapse series of wt and clp1
cells expressing the nucleolar marker Nuc1p-GFP and the spindle pole body marker Cdc11p-GFP. Stacks of 11 z-sections of 0.5 µm were taken at 30-second intervals and projected as 2D images. Cells are shown at the indicated times. The first time SPBs labeled with Cdc11p-GFP appeared as separate dots was defined as time zero.