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Figure 4


Fig. 4. BMP-2 pre-treated neurosphere 24 hours after transplantation into the quail embryo neural tube (equal to stage 19, HH, total incubation time 72 hours). (A) Fluorescence image. (B) Enlargement of the boxed region in A. Emigrating SVZ neurosphere cells are visible by their typically stretched cellular morphology. Bilaterally emigrating cells are contained within the neuroepithelium (black borders of the neural tube). (C) In situ hybridization of the same embryo. Emigrated mouse SVZ cells can be seen in the roof plate of the neural tube when visualized using the mL1 probe. (D) Enlargement of C shows that only cell nuclei are stained. (E) Parallel section of embryo shown in C. Quail nuclei stained with the QCPN antibody, which recognises a species-specific antigen carried by all quail cells (brown colour), but not by mouse cells. A region of dorsal neural tube remains unstained. Immunohistochemistry for anti-GFP (F) shows that transplanted cells in the dorsal neural tube are GFP positive (mouse cells). Comparison with parallel sections in C, D, and E reveals that there is no cross-reactivity between mL1, QCPN and anti-GFP.





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