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Figure 1


Fig. 1. Isolation of a temperature-sensitive allele of tor2+. (A) Wild-type (tor2+), tor2ts (tor2-51) and the heterozygous diploid (tor2+/tor2-51) strains were spotted onto yeast extract (YES) medium at 1:10 dilutions. The plates were incubated at different temperatures (25, 30, 32 and 36°C). (B) Nomarski photomicrographs of wild-type (tor2+) and tor2ts (tor2-51) colony cells plated on YES medium after incubation overnight at 25°C, transferred to the indicated temperatures and then incubated for 12 hours. (C) Wild-type (tor2+) and tor2ts (tor2-51) cells were plated on YES medium and incubated at 36°C for three days (left panel). The same plate was then incubated at 25°C for a further three days (right panel). Photographs of the plates are shown.





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