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Figure 4


Fig. 4. Rum1 and Ste9 are required for G1 arrest but not for growth inhibition after Tor2 inactivation. (A) Flow cytometry analysis and Nomarski photomicrographs of h90 tor2+, h90 tor2-51, h90 tor2-51 rum1{Delta} and h90 tor2-51 ste9{Delta} cells incubated at 25°C or at 32°C for 8 hours and 24 hours, as shown. (B) The indicated strains were grown in EMM at 32°C for 24 hours. RNA was extracted and northern blot was performed and hybridised using probes against the ribosomal protein gene rps1102. The rRNA in each sample, stained with Methylene Blue, is shown to verify equal loading of RNA in each lane.





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