spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Surface and intracellular distribution of TfR1 and TfR2. (A) K562 cells were incubated at 4°C for 1 hour with either PBS (untreated cells, NT) or PBS containing trypsin (trypsinized cells, Tryps). For flow cytometry analysis, labeling with anti-TfR1 and anti-TfR2 was performed on intact cells and on fixed and permeabilized cells (fix-perm). (B-D) Aliquots of untreated (NT) and trypsinized (Tryps) K562 (B,D) or HepG2 (C) cells (4°C or 37°C) were lysed and total cellular lysates were resolved by SDS-PAGE and immunoblotted against TfR1, TfR2 and caveolin-1. Blots were stripped and reprobed for actin to ensure equal protein loading and transfer.





Right arrow Return to article