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Figure 10


Fig. 10. Effects of poly I:C on matrix protein synthesis and secretion. (A) Real time PCR. HASMCs were treated with or without 5 µg ml–1 poly I:C for 2 or 24 hours and relative mRNA expression levels using Taqman probes for elastin, versican and TSP1 were quantified using the comparative Ct method with HPRT as the internal standard. Expression data is normalized to that of untreated cells. The graph represents 2 separate experiments, each run in duplicate. All data are mean ± s.d. for quadruplicate determinations. (B) Effect of poly I:C treatment on accumulation of TSP1 in the medium of HASMC at the indicated times following transfer to serum free medium (closed bar) or the same medium containing 5 µg ml–1 poly I:C.





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