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Figure 4


Fig. 4. NoC1 binding to versican. Binding of [125I]-NoC1 to wells coated with the indicated concentrations of full-length versican (A) or VG1 (B) in buffers containing Ca2+ and Mg2+ (bullet) or 5 mM EDTA ({circ}) for 3 hours at 37°C. Background values were subtracted from each data point (365.8±28.7 and 286.0±21.3). (C) Binding of [125I]-TSP1 to wells coated using 1 µg per well of versican ({blacktriangledown}) or 0.3 µg per well of VG1 ({blacktriangleup}) was determined in the presence of the indicated concentrations of heparin. Binding was normalized to that determined in the absence of heparin (748.8±24.2 and 12658.8±117.4 cpm, respectively). All data are mean ± s.e.m. for quadruplicate determinations.





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