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Fig. 6. Distinct localization of extracellular hyaluronan and proteins induced by poly I:C. (A) Versican-rich matrix generated by inflammation is mostly distinct from the stress-induced hyaluronan-rich matrix. Image of versican (green, bottom left quarter) and hyaluronan (red, top right) are shown together with phase contrast image (top left) and merge (bottom right). Only limited co-localization of versican and hyaluronan was observed after stimulation with 5 µg ml–1 poly I:C for 17 hours. (B) Inflammation induces increased accumulation and co-localization of TSP1 with elastin. HASMC were cultured with 5 µg ml–1 poly I:C for 4 hours before being processed for immunofluorescence staining. Note that the anti-elastin antibody (green) binds to many of the same structures that are labeled with the anti-TSP1 antibody (red). Nuclei were counterstained with Hoechst 33258 (blue). Bars, 50 µm (A); 20 µm (B).
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