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Figure 9


Fig. 9. Heparin inhibits TLR3-induced deposition of exogenous TSP1. HASMC were cultured in standard medium (control) or in the presence of 5 µg ml–1 poly I:C for 15 hours, followed by an additional 1 hour incubation in culture medium containing 10 µg ml–1 TSP1 with or without the indicated concentrations of heparin. Staining with antibody against TSP1 showed that binding of exogenous protein to the structures generated by cells following poly I:C treatment was inhibited by heparin in a dose-dependent manner. Bar, 50 µm.





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