JCS03202 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 -

  Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Frozen sections from nestin-cre/Cx43^+/flox (A) and nestin-cre/Cx43^flox/flox (B) ciliary bodies were immunostained with the Sigma (main figures) and Invitrogen/Zymed (insets) anti-Cx43 antibodies. The pattern of loss of Cx43 from the pigment epithelium (PE) is also seen under these conditions. PC, posterior chamber.

• Supplemental Figure 2 -

  Fig. S2. Enophthalmos is visible in an 11-week-old Cx43^flox/flox (B) mouse but not in the Cx43^+/flox (A). (C, D) Following enucleation comparison of the control (lower, larger eyes) with the homozygous eyes (upper eyes) reveals miosis and a flaccid, buckling sclera.

• Supplemental Figure 3 -

  Fig. S3. Non-specific staining using the Alpha Diagnostics anti-Cx40 antibody. (A) Nestin-cre/Cx43^+/flox ciliary body. (B) Cx40^−/− ciliary body.