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Figure 10


Fig. 10. Tight junctions are functional at 2.5 weeks in the Cx43-deficient mice. The figure and inset B are from a nestin-cre+/Cx43+/flox animal, and inset A is from a nestin-cre+/Cx43flox/flox mouse. Five minutes following intravenous HRP injection, the reaction product can be seen by electron microscopy in stromal blood vessels of the ciliary body (BV), in the intercellular spaces between pigmented epithelial (PE) cells (white arrow) and at the interface between the (PE) and non-pigmented epithelium (NPE) (black arrows). In the insets, HRP diffusion can be seen blocked by tight junctions (TJ) so that no reaction product can be seen in the intercellular spaces either between the NPE cells (asterisks) or in the posterior chamber (PC). Bars, 500 nm.





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