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Figure 3


Fig. 3. Immunohistochemistry on paraffin sections of Cx43+/– (A) and Cx43–/– (B) eyes from E15 mouse embryos using the Sigma anti-Cx43 reagent. (A) In the heterozygote, strong signal is seen in the lens, ciliary body (CB), RPE (arrows, a), corneal epithelium (arrows, b). (B) In the homozygous Cx43 knockout, the lens staining persists, indicating a non-specific signal. The staining is completely removed, however, from the ciliary body, cornea and RPE.





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