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Figure 5


Fig. 5. Cx43 expression is abolished by nestin-cre in the pigmented epithelium of the ciliary body and retinal pigment epithelium of Cx43flox/flox mice. At P0 (B) and at P14 (D) a loss of detectable Cx43 staining is observed in the pigmented epithelium (PE) but not in the non-pigmented epithelium (NPE) of the ciliary processes in the nestin-cre+/Cx43flox/flox (B,D) as compared with the nestin-cre+/Cx43+/flox mice (A,C). Cx43 staining in the homozygote is also lost in the retinal pigment epithelium (arrows, A,B). There is no apparent change in Cx43 immunoreactivity in the lens due to the non-specific staining illustrated in Fig. 3. Bars, 50 µm (A,B); 20 µm (C,D).





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