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Fig. 9. Cx43 inactivation results in back-diffusion of plasma proteins from the veins into the eye chambers. (A) HRP reaction product stains the subendothelial connective tissue and vasculature in the stroma of the ciliary body (asterisk arrows) of a nestin-cre⁺/Cx43^+/flox mouse as visualized by light microscopy. The HRP is also visible in an iridal artery. (B) In a nestin-cre⁺/Cx43^flox/flox mouse, the HRP has entered the anterior chamber where H and E staining revealed a precipitate (see Fig. 6B and Fig. 7). HRP was contained in iridal blood vessels, notably in an indicated artery. Inset: electron microscopy demonstrates that the blood-ocular barrier at the level of the iridal (inset a) and retinal (inset b) blood vessels was not disrupted by Cx43 removal. These images show capillaries exhibiting containment of the HRP reaction product within the lumen of the vessel. The iridal vessel contains a peroxidase-positive erythrocyte. Bars, 20 µm (A); 10 µm (B); 500 nm (insets).

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