QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. Nef-induced CCL2/MCP-1 expression in astrocytes is dependent on calmodulin. U251MG-parental (P) and U251MG-Nef_Bru cells (Nef) were treated with 50 µM W7 (+) or solvent (–) for 16 hours. Total RNA was isolated and analyzed with Multiprobe^TM ribonuclease protection assay (A) and RT-PCR (B). The ratios of the CCL2/MCP-1 to GAPDH intensities were determined and the values obtained from the solvent-treated U251MG-Nef cells were set to 100%. One representative experiment of three independent experiments is shown. (C) Astrocytic U251MG-Nef_Bru, -pNeo and -parental cells were incubated for 8 hours with W7 at concentrations indicated, and CCL2/MCP-1 protein in the supernatants was determined by ELISA. One representative experiment is shown. Data represent mean ± s.e.m. of duplicates.

Return to article