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Fig. 4. Subcellular localization of wild-type and mutant podoplanin proteins. Confocal images of horizontal (x-y) and vertical (x-z) sections of MDCK cells expressing the indicated podoplanin proteins fused to EGFP are shown. The exogenous proteins specifically expressed at the cell surface were detected by in vivo pre-embedding staining using antibodies either recognizing the podoplanin EC domain or EGFP (for detection of P
EC).