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Figure 7


Fig. 7. The sensitivity to oxidising agents observed in the grx3grx4 double mutant is partly mediated by Aft1. (A) Cells from exponentially growing cultures of wild-type, grx3grx4, aft1 and grx3grx4aft1 strains were serial diluted and spotted onto SD plates either containing or not oxidising agents as in Fig. 1. (B) Wild-type and grx3grx4 strains growing exponentially were treated with 2 mM ferrocene for 6 hours, after which cells were washed and placed in fresh medium to be subsequently serial diluted and plated on SD plates containing 1 mM hydrogen peroxide or 1 mM hydrogen peroxide plus 150 µM ferrocene (F).





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