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Figure 8


Fig. 8. Aft1 nuclear localisation is regulated by both Grx3 and Grx4. (A) GFP-Aft1 fusion protein was visualised by fluorescence microscopy and recorded in different backgrounds. 1. wild type. 2. grx3grx4 double mutant. 3. grx3grx4 double mutant overexpressing GRX3 under the tetO7 promoter. 4. grx3grx4 double mutant overexpressing GRX4 under the tetO7 promoter. (B) Northern blot of FET3 from mRNA samples collected from the same experiment described in A. To regulate gene expression under the tetO7 promoter, we added (+) or not (–) 20 µg/ml doxycycline to the culture media.





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