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Figure 1


Fig. 1. Localization of EMILIN1 in first-trimester human placenta. (A) Cryostat sections of first-trimester placenta were stained with antibodies specific for EMILIN1 followed by secondary HRP-labeled antibodies and developed with DAB. The sections were counterstained with hemallum. (B) This section was immunostained for EMILIN1 (in brown) and for the presence of vWF, visualized with an alkaline-phosphatase-labeled secondary antibody, and it was not counterstained. Asterisk indicates a modified vessel; lower panel: triple staining for EMILIN1 (in brown), and cytokeratin (in green fluorescence) and vWF (in red) to visualize EVT and endothelial cells, respectively. (C) Cryostat sections of first-trimester placenta were immunostained for EMILIN1 and for the presence of vWF. Upper panel: EMILIN1 (in brown) and vWF (in pink); lower panel: EMILIN1 (in pink) and vWF (in brown). Arrowheads indicate spiral arteries and asterisks large modified vessels where the gradient distribution of EMILIN1 seems more evident. Bars, 50 µm.





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