QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. TAp63 isoforms bind and transactivate the IKK ${alpha}$ promoter. (A) Schematic structure of the IKK ${alpha}$ promoter showing the different putative p53-like, Ets-1 and GATA-3 responsive elements. (B) Luciferase assay analysis showing the ability of TAp63 ${alpha}$ to induce IKK ${alpha}$ expression. The ratios of IKK ${alpha}$ promoter to transcription factor are: IKK ${alpha}$ :TAp63 ${alpha}$ / ${Delta}$ Np63 ${alpha}$ , 1:0.5; IKK ${alpha}$ :Ets-1, 1:2; IKK ${alpha}$ :TAp63 ${alpha}$ / ${Delta}$ Np63 ${alpha}$ :Ets-1, 1:2:2. The luciferase assay shown was performed in Saos-2 cells, and similar results were also obtained in HEK293 cells (data not shown). Results are shown as mean ± s.d. of three independent experiments. (C) Luciferase assay analysis showing the ability of TAp63 and ${Delta}$ Np63 isoforms to transactivate IKK ${alpha}$ in Saos-2 cells. Results are similar to those obtained in Saos-2 Tet-on inducible cells in B. The ratio of IKK ${alpha}$ promoter to TAp63/ ${Delta}$ Np63 isoforms was 1:2. Results are shown as mean ± s.d. of three independent experiments. (D) ChIP showing the binding of p63 protein to the IKK ${alpha}$ promoter. Lane 1, marker; lane 2, specific antibody (anti-HA); lane 3, non-specific antibody (nsp); lane 4, input+Dox; lane 5, input–Dox, (representative result of two independent experiments is shown). (E) Real-time PCR performed on Dox (Tet-on) inducible Saos-2 clones. TAp63 isoforms already induce a significant increase of IKK ${alpha}$ RNA upon 24 hours of Dox treatment, whereas ${Delta}$ Np63 ${alpha}$ and p53 do not significantly change IKK ${alpha}$ RNA levels. Results are mean ± s.d. of three independent experiments.

Return to article