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Figure 1


Fig. 1. Expression and localization of PrPC in brain blood vessels. Transmission and immunofluorescence labeling of PrPC and ZO-1 of brain sections from wild-type (WT) and PrPC-deficient knockout (KO) mice. Sections of striatum were incubated with anti-PrPC monoclonal antibodies (SAF32 plus SAF61, both at 1 µg/ml) and anti-ZO-1 polyclonal antibodies (5 µg/ml) for 16 hours at 4°C, then with Alexa Fluor 488-conjugated anti-mouse antibodies and Cy3-conjugated anti-rabbit antibodies for 45 minutes at 25°C. Images were collected with a confocal fluorescence microscope. Arrows in the left panel indicate blood vessels; insets show microvessels (chosen from a different field). Bars, 40 µm or 10 µm (inset).





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