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Figure 11


Fig. 11. PrPC expressed by monocytes and endothelial cells is involved in transmigration of freshly isolated monocytes. Freshly isolated monocytes were labeled as above and separately incubated (monocytes) with anti-CD71, anti-PECAM-1 or anti-PrPC antibodies at 20 µg/ml for 1 hour, as indicated. Similarly, hCMEC/D3 endothelial cells, grown and activated as above, were separately incubated in the same conditions (hCMEC/D3). In parallel, both monocytes and hCMEC/D3 endothelial cells (monocytes + hCMEC/D3) were separately incubated in the same conditions. Monocytes were then added to the apical compartments of Transwell filters and allowed to transmigrate for 3 hours in the presence of MCP-1 (25 ng/ml) in basal compartments, as above. Average values (± s.e.m.) from one representative experiment out of three independent experiments are presented. The number of migrated cells following incubation with CD71 antibodies was taken as 100% in each condition (*P<0.01 versus cells pre-treated with anti-CD71 antibody).





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