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Figure 2


Fig. 2. Expression of PrPC at the cell-cell contacts of primary brain endothelial cells. Immunofluorescence labeling of PrPC, PECAM-1 and ZO-1 in freshly isolated brain microvessels. Cells were incubated with anti-PrPC (SAF32, 2 µg/ml), anti-PECAM-1 (3A12, 2 µg/ml) or anti-ZO-1 (1 µg/ml) antibodies for 16 hours at 4°C, then with Cy2-conjugated anti-mouse antibodies or Cy3-conjugated anti-rabbit antibodies for 45 minutes at 25°C. Images were collected with a confocal fluorescence microscope. Bars, 20 µm (A, xy), 10 µm (A, z), 10 µm (B).





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