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Figure 2


Fig. 2. Distribution of histone H3K4me2 in S. ocellaris embryonic germ cell nuclei at the resting stage. (A-C) Chromosome staining with DAPI, (A'-C') indirect immunolabelling with anti-H3K4me2 antibody and (A"-C") superimposed images with antibody staining in red. (A) S. ocellaris germ nuclei before, (B) during and (C) after the occurrence of Xp-chromosome elimination. In all cases only four chromosomes of the complement are strongly labelled (A"-C"). (B-B") A S. ocellaris germ nucleus undergoing elimination; the Xp chromosome is expelled from the nucleus (asterisk in B) and is not stained (asterisks in B' and B"). Bar, 10 µm.





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