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Figure 5


Fig. 5. Distribution of histone H3K4me2 in S. ocellaris embryos at the early syncitial stage. (A-C) chromatin DAPI staining, (A'-C') indirect immunolabelling with anti-H3K4me2 antibody and (B",C") superimposed images where antibody staining is in red. (A) A young whole embryo showing that the germ nuclei at the polar region of the embryo (arrows in A) are devoid of labelling (arrows in A'). (B-B") Enlarged images of the same embryo where it is seen that the antibody labelling is restricted to the somatic mitotic chromosomes whereas germ nuclei at interphase (arrowheads in B) remain unstained. (C-C") Partial view of the polar region of an embryo showing dividing germ cells (arrows in C) lacking antibody staining in contrast with somatic nuclei at interphase that display H3K4me2 staining. Bars, 10 µm.





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