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Figure 6


Fig. 6. Distribution of methylated histones in S. ocellaris spermatocytes undergoing paternal chromosomes elimination at first meiotic division (A-C) and in spermatids (D,E). (A-C) DAPI-staining of anaphase-like stage figures. m, maternal group of chromosomes; p, paternal chromosomes; * indicates position of the single spindle pole. (D,E) DAPI staining of (D) early and (E) late spermatids. (A'-E') Indirect immunolabelling with antibodies against histones H3 and H4 as indicated, and (A"-E") superimposed images where the antibody staining is in red. At first meiosis (A"-C"), the four maternal chromosomes that face the pole are devoid of staining whereas the opposed paternal group of chromosomes, closely grouped, show antibody labelling. During spermiogenesis (D,E), antibody labelling is observed in the eliminated paternal chromatin. Bars, 10 µm.





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