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Figure 7


Fig. 7. Distribution of RNA pol II in S. ocellaris embryonic germ nuclei at the resting stage (A,C) and male larva germ nucleus at 5 days from hatching (B). (A-C) Chromosome DAPI staining. (A'-C') Indirect immunolabelling with antibody against RNA pol II (red in C') and double staining with antibody against H3K4me2 (green in C"). (A",B") superimposed images where RNA pol II staining is in red and (C"') merged image where RNA pol II is in red and H3K4me2 in green. (A-A") Prior to the occurrence of Xp chromosome elimination (nine chromosomes), RNA pol II is observed in four chromosomes of the complement. (B-B") RNA pol II staining is restricted to the condensed maternal chromosomes (arrows in B) whereas decondensed paternal chromosomes are devoid of staining. (C-C"') A germ cell nucleus after the occurrence of Xp chromosome elimination (8 chromosomes), showing that labelling of RNA pol II and H3K4me2 is present in the same chromosomes of the complement. Bar, 10 µm.





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