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Figure 8


Fig. 8. Distribution of RNA pol II CTD phosphorylated on Ser2 in S. ocellaris somatic and germline nuclei of an embryo at 72 hours of development (A) and in male premeiotic nuclei and spermatocytes at first meiotic division undergoing paternal chromosomes elimination. m, maternal group of chromosomes; p, paternal chromosomes (B). (A,B) Chromatin DAPI staining, (A',B') indirect immunolabelling with anti-pSer2 CTD RNA pol II antibody and (A",B") superimposed images where antibody staining is in red. (A) A somatic nucleus (arrow) staining positive whereas a germ nucleus at the resting stage is devoid of staining. (B) Partial view of a testis cyst showing that premeiotic germ nuclei (arrows) are clearly stained whereas no staining is detected during meiosis I on both maternal and paternal chromosomes. Bars, 10 µm.





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