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Figure 3


Fig. 3. Immunohistochemistry of mouse small intestine. (A) Overview of FATP4 distribution. FATP4 (green) is mostly above the nuclei (blue) and oriented towards the lumen of the gut. Intestinal villi are shown in midsection. Bar, 40 µm. (B) Comparison of FATP4 and an apical marker protein. Sections were labeled with affinity purified antibodies against FATP4 and a mouse monoclonal antibody against PLAP. Pre-incubation with the C-terminal peptide of FATP4 abolishes the cytoplasmic staining pattern of FATP4. Exposure times for sections were identical. Bar, 10 µm. (C) Analysis of intestinal lysates of embryos of wt and FATP4 KO mice. Equal amounts of total lysate (50 µg) were separated by SDS-PAGE and developed by western blotting. A strong band at 72 kDa indicates the presence of FATP4 in the wt but the absence in the intestine of KO mice. Molecular mass markers are indicated in kDa.





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