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Figure 2


Fig. 2. Preferential expression of GRIF1 mRNA and protein in brain and heart. (A) Northern blot analysis of GRIF1 mRNA expression. A rat multi-tissue northern blot was hybridized with a 32P-labeled partial cDNA probe of GRIF1 (top). Equal loading was confirmed by using a ß-actin cDNA probe (bottom). Sk., Skeletal. (B) Specificity of anti-GRIF1 antibodies, GF1 and GF2. Western blot analysis of cell lysates from untransfected PC12 and HeLa cells, and pCHA-GRIF1-transfected HeLa cells using anti-GRIF1 antibodies GF1, GF2, or the corresponding pre-immune serum. The asterisks indicate non-specific immunoreactive bands, and the open arrowheads indicate bands that appear specific to the GF2 antibody. (C) Equal amounts of homogenates (100 µg) from the indicated rat tissues were analyzed by immunoblotting using anti-GRIF1 (GF1) and anti-ß-actin antibodies. (D) NGF-differentiated PC12 cells were immunostained using purified anti-GRIF1 antibodies GF1 or GF2 (green); Hoechst-stained nuclei (blue). Insets: enlarged views of boxed region showing punctate staining. Bars, 10 µm.





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