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Figure 3


Fig. 3. GRIF1 and Hrs associate in vivo. (A) Co-immunoprecipitation of GRIF1 with Hrs in transfected HeLa cells. HeLa cells were co-transfected with pEGFP-GRIF1 in combination with pCHA-Hrs or pCHA-vector. Lysates were immunoprecipitated with anti-HA antibody, followed by immunoblotting with anti-GFP and anti-HA antibodies. (B) Co-immunoprecipitation of endogenous Hrs with GRIF1 in PC12 cells. PC12 cell lysates were immunoprecipitated with anti-GRIF1 antibody or pre-immune serum, followed by immunoblotting for Hrs and GRIF1.





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