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Figure 6


Fig. 6. GRIF1 is recruited to enlarged early endosomes in Hrs-overexpressing cells. HeLa cells were transfected with pCHA-GRIF1 alone (A) or co-transfected with pEGFP-Hrs (B). HA-GRIF1 was detected using an anti-HA antibody (red). Endogenous Hrs was detected using an anti-Hrs antibody (green; A), and GFP-Hrs was visualized by the green fluorescence emitted by the GFP tag (green; B). Bars, 10 µm.





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