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Fig. 7. GRIF1 overexpression alters the distribution of early endosomes. HeLa cells were transiently transfected with pEGFP-GRIF1 (A), pEGFP-GRIF1
1 (B), pEGFP-GRIF1
2 (C), pEGFP-GRIF1
3 (D), or pEGFP-GRIF1
4 (E). GFP-GRIF1 was visualized by the green fluorescence emitted by the GFP tag (green), and endogenous EEA1 was detected using an anti-EEA1 antibody (red). Bars, 10 µm.