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Figure 5


Fig. 5. A23187 induces the translocation of PKC{alpha} to the membrane. (A) Cytosolic and membrane fractions were prepared from HEK293 reporter cells treated with vehicle (DMSO) or A23187 (2.5 µM) in the presence or absence of Wnt3a-CM, for western blotting with anti-PKC{alpha} antibody. The blots were reprobed with anti-actin or anti-MDR antibodies as fractionation controls. (B) The cellular location of PKC{alpha} in HEK293 reporter cells was determined by immunofluorescence analysis. After fixation, the cells were stained with anti-PKC{alpha} antibody and observed at 400x magnification. Arrows indicate PKC{alpha} translocated to the membrane.





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