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Figure 8


Fig. 8. Inhibition of PKC partially abolished the Wnt5a-mediated CRT inhibition. (A) HEK293 reporter and control cells were co-transfected with Wnt3a (50 ng) and Wnt5a [0.2 µg (+) and 1 µg (++)] plasmids for 48 hours, and then luciferase activity was determined. (B) HEK293 reporter and control cells were co-transfected with Wnt3a (50 ng) and Wnt5a (1 µg) plasmids, incubated with BIM [2.5 µM (+) and 5 µM (++)] for 15 hours, and then luciferase activity was measured. In A and B, the results shown are the average of three experiments; the bars indicated standard deviations.





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