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Figure 1


Fig. 1. Cortical mid1-4GFP expands towards the non-growing cell end in pom1{Delta} mutants. (A) GFP fluorescence in living wild-type (left panel-AP 1442) and pom1{Delta} cells (right panel-AP 1502) expressing mid1-4GFP, grown at 25°C. Bar, 5 µm. (B) GFP (upper panel) and fluostain I fluorescence (lower panel) in living pom1{Delta} cells expressing mid1-4GFP. Note that mid1-4GFP is present at the non-growing tip. Bar, 5 µm. (C) Distribution of mid1-4GFP in highly elongated pom1{Delta} cells obtained by treatment with 11 mM HU for 5 hours at 25°C. Note that HU treatment increases the pool of nuclear mid1-4GFP. mid1-4GFP spots are less abundant in the tip region compared with the medial or lateral cortex. Bar, 5 µm. (D) mid1-4GFP fluorescence profile along the cortex in wild-type (left panel) and pom1{Delta} cells (middle and right panels). Cortical intensity of mid1-4GFP was measured clockwise along the entire cell cortex of cells shown on the bottom, starting at the left cell tip. Dotted lines represent the position of the right cell tip. (E) Distribution of mid1-4GFP cortical spots in wild-type and pom1{Delta} cells. 223 wild-type and 268 pom1{Delta} cells from three independent experiments were analyzed. The presence of one or more mid1-4GFP spots at the medial (a), lateral (b) and tip (c) cortex (see scheme on the right) was scored in each cell, and correlated to cell length to monitor cell-cycle progression. Typical a-, abc- and ab-cells are shown in D (from left to right).





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