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Fig. 6. Automated analysis of the distribution of mid1-4GFP cortical spots. (A) After manual selection (a), and determination of cell medial and long axes by ellipse fitting (b), coordinates of cortical spots (c) and nuclei (d) on cell axes were automatically extracted. (B) Distribution of distances between cortical spots (left) or spots centroids (right) and the medial axis in wild-type (n=107), bud6 ${Delta}$ (n=96), tea3 ${Delta}$ (n=95), tea1 ${Delta}$ (n=103), pom1 ${Delta}$ (n=77) and pom1 ${Delta}$ tea3 ${Delta}$ (n=94) cells expressing mid1-4GFP, grown at 25°C. Spots centroids represent the mean distribution point of spots in individual cells. (C) Percentage of off-set spots (2 µm apart from the medial axis or more) or spots centroids (0.75 µm apart from the medial axis or more) in wild-type (1), bud6 ${Delta}$ (2), tea3 ${Delta}$ (3), tea1 ${Delta}$ (4), pom1 ${Delta}$ (5) and pom1 ${Delta}$ tea3 ${Delta}$ (6) cells expressing mid1-4GFP, grown at 25°C.

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