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Fig. 1. Cardiac injury in newts results in a transient downregulation of contractile proteins. Immunofluorescence staining (green) of sham operated (A,B) and damaged newt hearts (C-F) for MyHC (A,C,E) and cTnT (B,D,F) 1 (C,D) and 14 days (E,F) after mechanical injury. The white dashed lines in C and D indicate the margins of the hearts. Note the massive and widespread downregulation of MyHC and cTnT, which corresponded to the degree of damage exerted to the heart. 14 days after the injury cardiomyocytes in the injured areas re-expressed MyHC and cTnT (E,F). A Hoechst 33342 counterstaining was used to label all nuclei on the sections.