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Fig. 3. Cardiomyocytes maintain the expression of heart-muscle-specific genes after transplantation into intact limbs and under culture conditions. (A,B,D,E,G,H) Stained cryosections. DiI-labeled cardiomyocytes (red) transplanted into intact limb maintained the expression of the cardiac-muscle-specific cTnT (A,B,G,H; green). (C,F,I) Newt cardiomyocytes in culture expressed (C) cTnT and (F,I) MyHC (MF20-staining). Sections and cultures were stained with Hoechst 33342 (blue) to visualize all nuclei.