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Figure 5


Fig. 5. Erv41p and Erv46p co-sediment on a sucrose velocity gradient. A post-nuclear supernatant (T) of FY834 cells was solubilized in 1% Triton X-100 (S) and separated on a 5-19% sucrose gradient. Fractions were collected from the top and blotted for Erv41p and Erv46p, as well as for Erv25p, which is present in a 100 kDa complex that serves as a molecular mass marker. Relative amounts of these proteins were determined by densitometry of immunoblots and plotted. The position of the 440 kDa marker ferritin was determined by spectrophotometry.





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