JCS03248 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Direct-current EF directs Dictyostelium cells to migrate directionally towards the cathode. On reversal of field polarity, the cells change direction to migrate towards the new cathode.

• Movie 2 -

  Movie 2. EF induces Ca²⁺_i spikes in Dictyostelium cells, whereas the cells migrate towards the cathode (to the right). EF 12 V/cm. Frames were taken at 15-second interval. Total duration of the video is 750 seconds. Warm colours (oranges and reds) represent elevated concentrations of Ca²⁺_i, whereas cooler colours (blues) represent lower levels of Ca²⁺_i.

• Movie 3 -

  Movie 3. iplA-null cells migrate directionally in an EF. This migration was reversed on reversal of field polarity.

• Movie 4 -

  Movie 4. Depletion of Ca²⁺_e completely abolished the electrotactic response, while cells migrate actively.