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Fig. 2. Siz proteins and Mms21 have overlapping functions. (A) Strains of the indicated genotypes were incubated on a YPD plate for 2 days at 30°C. Doubling times of mutants are indicated in parentheses. (B) Yeast strains of the indicated genotypes containing Net-His8 HA were analyzed as in Fig. 1, with immunoblotting with anti-Smt3 (top panel) or anti-HA (bottom panel). Designations are as in Fig. 1.