|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. Kinetics of transferrin and latex bead uptake in DCs immunolabelled for macropinosome-endosome markers: rabankyrin-5 and EEA1. This figure shows quantitative morphometric results (mean ± s.d. from at least ten fields each), obtained as described in Materials and Methods. Corresponding representative images and additional quantitative data with Dex and Bds are shown in supplementary material Fig. S2. Panel A compares the kinetics of uptake by DCs of two tracers, Tf and Bds, taken up by clathrin-dependent endocytosis and macropinocytosis, respectively. Notice that the first appears monophasic, whereas the second shows a considerable increase in rate after a few minutes of delay. Panel B illustrates the time course of Bd accumulation in puncta positive also for Rbk, EEA1 or both; panel C shows the accumulation of Tf in puncta positive for EEA1, and of Bds in puncta positive for Tf, EEA1 or both. Notice that the macropinocytic tracer becomes co-localized with the markers with a few minutes' delay with respect to Tf; and that its co-localization with the two markers is sequential: first Rbk-5 (newly generated macropinosomes and an early endosome subfraction), and then EEA1 (early endosomes).
|
