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Figure 3


Fig. 3. Macropinosome generation: dependence on PI3K and Ca2+, and effects of tracers on [Ca2+]i. Panels A-B show DCs loaded with Bds without pretreatments (A), after pretreatment with amiloride (B), after preloading with BAPTA (C), and after pretreatment with the PI3K-blocker drugs Wort (D) and LY-294002 (E) or the inactive analogue LY-303511 (F). Bar, 10 µm (B), also valid for A-D. Panels G and H show representative fura-2 ratiometric [Ca2+]i traces of DC suspensions exposed at the arrows to non-fluorescent Bds (G) or Dex (H). Panel I summarizes the average increases (results of four experiments ± s.d.) induced by the 5 minute addition of either tracer to DCs non-pretreated (NPT) or exposed for the same time to Bds after pretreatment with Wort, Vac1 or BAPTA-AM.





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