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Figure 5


Fig. 5. A variety of mitotic spindle defects and lagging chromosomes in sub1/sub131 mutants stained for DNA (blue) and microtubules (green). Compared with (A) wild-type metaphase, sub mutant metaphase spindles are bipolar but exhibit several defects including (B) frayed microtubules (B', tubulin channel shown with increased levels), (C) asymmetric half spindles and (D) poorly oriented half spindles or absent interpolar microtubules. Although wild-type anaphase (E) (E', DNA channel only) involves the uniform segregation of chromosomes to the poles, in sub mutant anaphases there was an increased frequency of lagging chromosomes (F). A synergistic effect on spindle assembly was observed during (G) metaphase and (H) anaphase in sub1/sub131; polo16-1/+ mutants or metaphase (I) and (J) anaphase spindles in sub1/sub131;Incenp/+ mutants. Bars, 5 µm.





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