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Figure 6


Fig. 6. Subito and Polo co-immunoprecipitate. (A) HA-tagged Subito was immunoprecipitated from mixed stage 0- to 2-hour-old embryos and metaphase-arrested oocytes and then used for western blotting. (B) A control experiment was performed on tissue that did not express the HA-tagged Subito. (C) This experiment was repeated using GFP-tagged Polo (Moutinho-Santos et al., 1999). HA-tagged Subito was immunoprecipitated from mixed stage 0- to 2-hour-old embryos. (D) In the control, using embryos not expressing HA-tagged Subito, GFP-Polo was not precipitated. The lower band is a nonspecific protein in the lysate.





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