JCS03240 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Relationship between soluble tubulin and total tubulin under various conditions. (A) Effect of changing the stringency of nucleation. Note that when nucleation is stringent (red solid line), the curve looks very much like the classic curve shown in Fig. 3A. The dotted lines give the behavior of simulations performed when rescue is ‘turned off’ (occurs by setting to zero the probability of GTP tubulin adding to a GDP end). (B) Effect of rescue on the relationship between soluble tubulin and total tubulin in the presence (‘edge on’) and absence (‘edge off’) of spatial confinement. Note that the shape of the blue line (no rescue, no spatial confinement) is very similar to that predicted by Mitchison and Kirschner (Mitchison and Kirschner, 1987).

• Supplemental Figure 2 -

  Fig. S2. Impact of changes in nucleating activity. Relationship in a spatially confined system between the number of nucleation sites and either the average MT length (A) or free tubulin concentration (B). All other parameters are identical for all simulations represented in this figure (the cell size and all other parameters are the same as used for the simulations analyzed in Table 1).

• Movie 1 -

  Movie 1. Movie of rectangular cell like that shown in Fig. 1

• Movie 2 -

  Movie 2. Movie of radial cell, low concentration of tubulin (in vitro-like); corresponds to ‘in vitro-like’ of Table 1

• Movie 3 -

  Movie 3. Movie of radial cell, high concentration of tubulin, 1×MT; corresponds to ‘in vivo-like, interphase’ of Table 1

• Movie 4 -

  Movie 4. Movie of radial cell, high concentration of tubulin, 4×MT; corresponds to ‘in vivo-like, mitosis’ of Table 1