spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Relationships between total tubulin and soluble tubulin ([Tu]soluble) at steady-state. (A) Classically expected behavior. Note that little polymer is seen until the critical concentration (Cc) is achieved. At total tubulin concentrations above Cc, all additional tubulin is incorporated into polymer, and the concentration of unpolymerized tubulin remains at Cc. See Materials and Methods for the equations used to plot these curves. (B) Relationship observed in our simulations. Solid red line: system without spatial confinement. Dashed red line: system with spatial confinement. The dotted grey line gives Cc (i.e. [Tu]soluble) that is asymptotically approached as [Tu]total increases. Notice that, in confined systems there is no easily observed Cc. Instead, the concentration of free tubulin continues to rise as total tubulin rises, at first slowly, and then more steeply. The curves in B are not fits to an equation but are provided to guide the eye as it follows the progression of the data.





Right arrow Return to article