JCS03274 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 -

  Table S1. Primary screen results. This list gives Genepairs ID for each gene that scored positive in the primary screen. Each of these genes was subsequently analyzed by Nomarski microscopy.

• Supplemental Table 2 -

  Table S2. Functional information and homologs. Sequence name, genepairs ID, locus, Ahringer autoclass assignments (column 4) (Kamath et al., 2003) and functional class assignments used in this study (column 5) are listed for each DTC migration gene. The Drosophila (column 6) and Human (column 7) homologs, the InterPro Molecular Functions (column 8), and the NCBI KOG information (column 9) listed here are all available from WormBase Freeze WS140 (www.wormbase.org). *Molecular Functions gleaned from the literature, not from InterPro.

• Supplemental Table 3 -

  Table S3. DTC migration data, statistical analysis, and phenotypic characterization. This table includes the information from Table 1 plus the 95% confidence intervals (CI) and the percentages of Type 1, 2, and 3 defects for each RNAi experiment and for P0 and F1 animals. Sequence name, Genepairs ID, and genetic locus are indicated. The CI specifies a range of values within which the mean lies with 95% confidence and is calculated based on a binomial distribution Beyer, W. H. (1968) CRC handbook of tables for probability and statistics. Cleveland, OH: The Chemical Rubber Company. The high and low 95% confidence limits for each measurement are listed in the blue columns. In cases where the sum of percentages in Types 1-3 does not total 100%, the remaining percentage of animals had no gonad arm. Black boxes in the final two columns indicate those genes that have been shown to be expressed in the distal tip cell (DTC) and/or body wall muscle (BWM). Blank boxes, no expression in DTC or BWM, or no data available. Data are from www.wormbase.org and from the British Columbia C. elegans Gene Expression Consortium database.

• Supplemental Table 4 -

  Table S4. Primers used to amplify RNAi targeting inserts. Sequence name, locus, and primer sequence are given for constructs prepared for this study. The numbers indicate the positions in the cDNA sequence. F, forward; R, reverse.

• Supplemental Figure 1 -

  Fig. S1. DTC migration is controlled by a network of gene interactions. Red nodes represent the genes required for DTC migration isolated in this screen. Green nodes are a subset of interacting genes derived from a genome wide prediction of C. elegans genetic interactions (Zhong and Sternberg, 2006). This network is identical to Fig. 5 except that here all nodes are identified.