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Figure 1


Fig. 1. Two-step screen to identify DTC migration defects. (A) Nematodes were cultured on bacterial strains from hatching to adulthood. The primary screen was performed 48 hours post hatching and young adults were scored for the presence of clear patches using a dissecting microscope. In the secondary screen, DTC migration paths were documented by Nomarski microscopy. (B) Diagram illustrating one arm of the hermaphrodite gonad. The DTC is indicated. Cuboidal cells are maturing oocytes. Ovals are fertilized embryos. (C-G) Nomarski images showing three types of defects. Treated rrf-3(pk1426) hermaphrodites were grown on E. coli HT115(DE3) carrying an empty vector (C) or RNAi targeting vectors for act-1 (D), ced-5 (E), pat-3 (F), or dyn-1 (G). Arrows indicate paths taken by DTCs. Anterior is to the left, ventral down in all images except D and F in which anterior is to the right. Bars, 20 µm.





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